Resistance to Tecia solanivora (Lepidoptera: Gelechiidae) in Three Transgenic Andean Varieties of Potato Expressing Bacillus thuringiensis Cry1Ac Protein

2007 ◽  
Vol 100 (1) ◽  
pp. 172-179 ◽  
Author(s):  
Ana Milena Valderrama ◽  
Nubia Velásquez ◽  
Esperanza Rodríguez ◽  
Andrea Zapata ◽  
Mohsin Abbas Zaidi ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Tecia Solanivora ◽  
Cry1ac Protein

Resistance to Tecia solanivora (Lepidoptera: Gelechiidae) in Three Transgenic Andean Varieties of Potato Expressing Bacillus thuringiensis Cry1Ac Protein

2007 ◽  
Vol 100 (1) ◽  
pp. 172-179
Author(s):  
Ana Milena Valderrama ◽  
Nubia Velásquez ◽  
Esperanza Rodríguez ◽  
Andrea Zapata ◽  
Mohsin Abbas Zaidi ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Tecia Solanivora ◽  
Cry1ac Protein

scholarly journals Bacillus thuringiensis Bel Protein Enhances the Toxicity of Cry1Ac Protein to Helicoverpa armigera Larvae by Degrading Insect Intestinal Mucin

2009 ◽  
Vol 75 (16) ◽  
pp. 5237-5243 ◽  
Author(s):  
Shangling Fang ◽  
Li Wang ◽  
Wei Guo ◽  
Xia Zhang ◽  
Donghai Peng ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Trichoplusia Ni ◽  
Knockout Mutant ◽  
Gene Encoding ◽  
In Vitro Degradation ◽  
Intestinal Mucin ◽  
Helicoverpa Armígera ◽  
Cry1ac Protein

ABSTRACT Bacillus thuringiensis has been used as a bioinsecticide to control agricultural insects. Bacillus cereus group genomes were found to have a Bacillus enhancin-like (bel) gene, encoding a peptide with 20 to 30% identity to viral enhancin protein, which can enhance viral infection by degradation of the peritrophic matrix (PM) of the insect midgut. In this study, the bel gene was found to have an activity similar to that of the viral enhancin gene. A bel knockout mutant was constructed by using a plasmid-free B. thuringiensis derivative, BMB171. The 50% lethal concentrations of this mutant plus the cry1Ac insecticidal protein gene were about 5.8-fold higher than those of the BMB171 strain. When purified Bel was mixed with the Cry1Ac protein and fed to Helicoverpa armigera larvae, 3 μg/ml Cry1Ac alone induced 34.2% mortality. Meanwhile, the mortality rate rose to 74.4% when the same amount of Cry1Ac was mixed with 0.8 μg/ml of Bel. Microscopic observation showed a significant disruption detected on the midgut PM of H. armigera larvae after they were fed Bel. In vitro degradation assays showed that Bel digested the intestinal mucin (IIM) of Trichoplusia ni and H. armigera larvae to various degrading products, similar to findings for viral enhancin. These results imply Bel toxicity enhancement depends on the destruction of midgut PM and IIM, similar to the case with viral enhancin. This discovery showed that Bel has the potential to enhance insecticidal activity of B. thuringiensis-based biopesticides and transgenic crops.

scholarly journals Actividad biológica de Bacillus thuringiensis sobre la polilla guatemalteca de la papa, Tecia solanivora Povolny (Lepidoptera: Gelechiidae)

Revista Mutis ◽  
2013 ◽  
Vol 3 (2) ◽  
pp. 31-42
Author(s):  
Adriana Carolina Rojas Arias ◽  
Silvio Alejandro López Pazos ◽  
Alejandro Chaparro Giraldo
Keyword(s):  
Solanum Tuberosum ◽  
Bacillus Thuringiensis ◽  
Tecia Solanivora

La papa (Solanum tuberosum) es uno de los cultivos más importantes de Colombia. Las larvas de la polilla guatemalteca de la papa, Tecia solanivora Povolny (Lepidoptera: Gelechiidae), causan daños directos a los tubérculos, produciendo pérdidas económicas e incremento en el uso de agroquímicos. Bacillus thuringiensis (Bt) es una alternativa en el manejo de insectos plaga gracias a su especificidad. Su actividad depende de proteínas denominadas Cry, que cuando son ingeridas por un insecto susceptible forman poros en sus células intestinales que producen la muerte del insecto. Esta revisión presenta los estudios sobre el efecto de Bt hacia T. solanivora. Se ha encontrado que las toxinas Cry1Ac (base para variedades transgénicas) y Cry1B tienen importante actividad tóxica. Igualmente se destaca la experiencia del diseño y evaluación de una toxina híbrida (Cry1B-Cry1I) que resultó en una importante letalidad hacia T. solanivora.

scholarly journals Principales microorganismos entomopatógenos de Tecia solanivora y sus mecanismos de acción

2021 ◽  
Author(s):  
Emmanuel Guerra-Luran ◽  
Jovanna Acero-Godoy
Keyword(s):  
Solanum Tuberosum ◽  
Bacillus Thuringiensis ◽  
Tecia Solanivora

La papa (Solanum tuberosum) es uno de los cultivo de mayor importancia en Colombia y a nivel mundial, se destaca por ser una de las fuentes vegetales de proteínas y carbohidratos. S. tuberosum se ve afectado por una gran cantidad de plagas, entre ellas se encuentra Tecia solanivora, este importante fitopatógeno fue identificado en Guatemala - (Centroamérica). El presente artículo está centrado en exponer el control biológico utilizado para contrarrestar el ataque de T. solanivora, que abarca el uso de microorganismos y la producción de exo-productos microbianos. Actualmente los principales microorganismos antagónicos de T. solanivora son: Bacillus thuringiensis (Bt), granulovirus, y los nematodos Steinernema sp y Heterorhabditis sp. Para lograr la infección, estos microorganismos deben sobrevivir las condiciones adversas del ambiente, colonizar su entorno y finalmente evadir la respuesta inmune del huésped, lo anterior lo logran produciendo diferentes exo-productos con actividades antimicrobianas, líticas y tóxicas, entre otros. Esta importante característica le permite a un agricultor mantener las poblaciones del insecto a niveles inferiores.

High Sensitivity Bacillus thuringiensis Cry1Ac Protein Detections Using Fluorescein Diacetate Nanoparticles

2015 ◽  
Vol 26 (2) ◽  
pp. 451-457 ◽  
Author(s):  
Cui Liu ◽  
Zhen Zhou ◽  
Linling Zou ◽  
Yuan-Cheng Cao ◽  
Jun’An Liu ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
High Sensitivity ◽  
Fluorescein Diacetate ◽  
Cry1ac Protein

scholarly journals Characterization of the mucosal and systemic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD 73 in mice

2000 ◽  
Vol 33 (2) ◽  
pp. 147-155 ◽  
Author(s):  
R.I. Vázquez-Padrón ◽  
L. Moreno-Fierros ◽  
L. Neri-Bazán ◽  
A.F. Martínez-Gil ◽  
G.A. de-la-Riva ◽  
...  
Keyword(s):  
Immune Response ◽  
Bacillus Thuringiensis ◽  
Systemic Immune Response ◽  
Cry1ac Protein

Selective Feeding of Soybean Looper (Lepidoptera: Noctuidae) on Meridic Diet with Different Concentrations of the Bacillus thuringiensis Cry1Ac Protein

2004 ◽  
Vol 39 (2) ◽  
pp. 243-250
Author(s):  
Jeffrey Gore ◽  
John J. Adamczyk
Keyword(s):  
Bacillus Thuringiensis ◽  
The Other ◽  
Pseudoplusia Includens ◽  
Selective Feeding ◽  
Choice Assay ◽  
Soybean Looper ◽  
Lepidoptera Noctuidae ◽  
Cry1ac Protein ◽  
Meridic Diet

The ability of soybean loopers, Pseudoplusia includens, (Walker), to selectively feed on meridic diet with different concentrations of Cry1Ac was investigated in a choice assay. Neonates selected non-treated meridic diet and diet with concentrations of Cry1Ac ranging from 0.1 to 1.0 μg/mL for feeding compared to diet with Cry1Ac concentrations of 5.0 and 10.0 μg/mL. Averaged across rating periods, means of 11.9 to 17.6% of larvae were observed feeding on diet with Cry1Ac concentrations ranging from 0.0 to 1.0 μg/ml, compared to 3.4% on diet with Cry1Ac concentrations of 5 μg/mL and 4.3% at 10 μg/mL. In a no-choice study, concentrations ranging from 0.1 to 1.0 μg/mL produced 4.3 to 40.8% mortality in soybean looper; while, concentrations of 5.0 μg/mL produced 58.5% mortality and 10.0 μg/mL produced 90.8% mortality. Also, diet with Cry1Ac concentrations of 5.0 and 10.0 μg/mL delayed the development of soybean looper larvae compared to diet with the other concentrations of Cry1Ac. Based on results of this study, soybean loopers can select diet with concentrations of Cry1Ac that result in low (<50%) levels of mortality.

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